Application

1. Synergy Taq has all the properties of regular Taq DNA Polymerases for every standard PCR application.

2. Optimized for high fidelity amplification of long template DNA with condensed GC-rich sequences; it can amplify up to 15 kb genomic DNA target and 35 kb lDNA sequence.

DESCRIPTION
Synergy Taq is a mix of thermostable polymerases possessing 5′-3′ DNA polymerase activity that is optimized to amplify longer GC-rich fragments and 3′-5′ proof-reading activity. These components are optimized to achieve amplification of 15 kb products from genomic DNA. Synergy Taq provides a very robust synthesis of longer GC-rich amplification products.

CONCENTRATION

10 units/µl

UNIT DEFINITION

One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-aminopropane-sulfonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCl, 2 mM MgCl₂, 1 mM .-mercaptoethanol) and activated calf thymus DNA as substrate.

STORAGE BUFFER

10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA; 1 mM DTT, 0.01% Tween 20; 50% glycerol (v/v).

STORAGE TEMPERATURE

Store Synergy Taq DNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.

10X REACTION BUFER

160 mM (NH)₄SO₄, 670 mM Tris-HCl pH 9,1; 8% Glycerol, 2% DMSO, 35 mM MgCl₂.

QUALITY CONTROL

Activity, non-specific endonucleases, nickases, and exonucleases.

COMPANION PRODUCTS

HotStart Synergy PCR Ready Mix, EU thin-wall PCR tubes, EU thin-wall 8-tube PCR strips, EU thin-wall 96-well PCR plates, MegaPure GeneClean Kit.